Project -> Consortium -> University of Oxford (UXOF)

3. University of Oxford (UXOF)

Partner Leaders : Hagan Bayley/Kalim Mir

1. Mir Group

Weblink : http://www.well.ox.ac.uk/nanogenomics/index.shtml

Tasks in project will include :

  • Development of multi-base and real-time ligation-based sequencing approaches.
  • Long Range single molecule genome analysis.
  • Developing microfluidics and imaging systems for the above tasks.
  • Single molecule approaches to methylation analysis.
  • Setting up microarray systems for single molecule analysis.
  • Investigation of methods for amplification in oil/water droplets and utilisation of nanopore sensing for mutation detection.

 

2. Bayley Group

Weblink : www.chem.ox.ac.uk/bayleygroup/

Professor Bayley's research interests lie in the exploration of membrane protein structure and function, and the use of molecular engineering techniques that transform these proteins into unique measurement systems or exploring the chemistry of individual molecules. Professor Bayley has studied the potential of nanopores to identify analytes at a single-molecule level. Recent publications from the Bayley laboratory have shown progress towards being able to use nanopores for the direct analysis of DNA, and the potential for fast and powerful genome sequencing using nanopores.

Tasks in project will include :

  • Exonuclease nanopore sequencing.
  • Integration of Protein Channels in Solid State Apertures.
  • Droplet-based bilayer arrays in miniaturised devices for clinical diagnostics.
  • Single Molecule Methlyometer.

 

3. Kapanidis Group

Weblink : http://www.physics.ox.ac.uk/users/kapanidis/group

Summary of our role in the consortium

Dr. Kapanidis has expertise in the characterisation of RNA and DNA polymerases by means of single molecule fluorescence spectroscopy and fluorescence resonance energy transfer. In particular, fluorescently labelled polymerase-nucleic acid complexes can be observed with high temporal and spatial resolution during processing of DNA. This expertise will be used in the READNA project to develop new methods of polymerase-based DNA sequencing and novel single-molecule coding schemes. This work is done in collaboration with Prof. Tom Brown (School of Chemistry, University of South Hampton) and Dr. Kalim Mir (Wellcome Trust Centre for Human Genetics, Oxford).