Workpackage 3: Fluorescence-based Single Molecule Sequencing

1. 'Multi-base sequencing by ligation'
   Development of a fluorescence-based method for bar-coding and higher convolution.
2. 'Real-time ligation-based sequencing-by-synthesis'
   Development of a procedure for adding, reading and removing labeled ligated entities in continuous acquisition.
3. 'Real-time polymerase based sequencing-by-synthesis: Quencher approach'
   Development of a nucleic acid reading system based on differential quenching of fluorescence by FRET.
4. 'Real-time polymerase based sequencing-by-synthesis: TWT approach'
   Development of a nucleic acid reading system based on traveling wave tracking.
5. 'Handling and imaging long genomic DNA fragments'
   Improvement of methods for stretching out nucleic acid molecules in nanochannels.
6. 'Contextual sequencing'
   Testing of procedures for nucleic acid interrogation on individual stretched nucleic acid molecules.